Quantification of the C3a fragment

Human plasma, serum, or other biological fluids

Sample collection is critical. Care must be taken to avoid C3a generation in the sample. For plasma, blood samples should be collected with disodium EDTA as anticoagulant and should be centrifuged at 2000xg at 2-8 °C.
The entire operation must be completed immediately. Samples should be prepared and assayed immediately or stored on ice for up to 2 hours.
For long-term storage freeze at -70 ºC with stabilizing solution.

Serum                71.0 – 589.2 ng/ml
EDTA Plasma     33.8 – 268.1 ng/ml

Human, cynomolgus macaque, baboon, rhesus macaque

Monoclonal mouse-antibody, specifically binds C3a-desArg.

The C3a enzyme immunoassay measures the amount of C3a-desArg in human EDTA plasma, serum and other research samples.
Under normal conditions, activation of the classical, alternativ or lectin complement pathways results in the formation of a C3 convertase muli-molecular enzyme capable of cleaving C3 to C3a and C3b. C3a is a low molecular weight (approximately 9 kD) protein fragment of 77 amino acids. C3a is rapidly metabolized by the serum enzyme , carboxypeptidase N, to the more stable, 76 amino acid form, C3a des-Arg.
The quantitation of C3a des-Arg therefore provides a reliable measurement of the level of complement activation in the test sample.

C3a, ELISA, C3a-Fragment, C3a-desArg, Complement activation, Biocompatibility, Hemocompatibility, Complement pathway, Complement cascade, ASTM F1984-99,
ASTM F2065-00